for the determination of biomolecular structures by x-ray crystallography,
NMR and cryoelectron microscopy is available in several departments.
X-ray crystallographic laboratories are in the Institute of Structural
Biology and Drug Discovery located in the Virginia Biotechnology
Research Park on the medical school campus. These consist of a dedicated
crystal growth and handling room connected to an x-ray laboratory
with two rotating anode x-ray generators. Data collection on site
is done with an MSC image plate detector on a rotating anode x-ray
source equipped with a focusing mirror system and cryocrystallography
attachment. A large number of graphics workstations are available
on a local area network, which also includes PCs, and is linked
to the VCU network server. An extensive library of molecular modelling
software is accessible from these workstations and in-house expertise
as well as formal courses in their use are available. Both 500mHz
and 300mHz NMR spectrometers are housed in dedicated labs on the
medical school campus and supervised by experienced faculty personnel.
Software and expertise in the determination of macromolecular structure
by multidimensional NMR are on site and make extensive use of the
molecular modelling facility. Fully equipped cryoelectron microscopy
facilities are in Sanger Hall on the medical school campus with
necessary software and workstations for data analysis. Biological
structure studies are coordinated with many investigators in different
departments whose projects require structural knowledge, and this
provides a fertile array of problems to be exploited through collaborations.