Luiz Shozo Ozaki, Ph.D.
Associate Professor

Phone: (804) 828-3897
Dept. Fax: (804) 828-9946
e-mail: lsozaki@vcu.edu

Address:
Department of Microbiology & Immunology
Virginia Commonwealth University
P.O. Box 980678
1101 East Marshall Street, 5-036 Sanger Hall
Richmond, VA 23298-0678

Professional Experience

• 2002 – present: Associate Professor, Virginia Commonwealth University , Richmond , VA
• 2001 (summer): Director of VCU Medicine and Life Science Scholars Program.
• 1997- 2002: Visiting Scholar, Virginia Commonwealth University , Richmond , VA
• 1997-1999: Senior Research Scientist, Commonwealth Biotechnologies, Inc., Richmond , VA
• 1997: Director (interim), Center of Biotechnology of Rio Grande do Sul at the University Federal of Rio Grande do Sul, Porto Alegre, RS, Brazil.
• 1995 - 1997: Adjunct Faculty, Washington State University, Pullman , WA
• 1988 - 1997: Associate Professor, University Federal of Rio Grande do Sul, Porto Alegre , RS, Brazil .
• 1987 - 1988: Associate Professor, Department of Parasitology, University of São Paulo, São Paulo, SP, Brazil.
• 1984-1987, Charge de Recherche, Institut Pasteur, Unite de Parasitologie Experimentale, Paris , France .

Research Interests

"Under Construction"

Selected Publications:

Nyhus KJ, Ozaki LS, Jacobson ES (2002). Role of mitochondrial carrier protein Mrs3/4 in iron acquisition and oxidative stress resistance of Cryptococcus neoformans. Med Mycol., 40(6):581-91.

Nicolaiewsky, T.B., Lunge, V.R., Cunha, C.W., Ikuta, N., Fonseca, A.S., da Silva, S.S., Richter, M.F., and Ozaki, L.S. (2001). Detection of Babesia equi (Laveran, 1901) by nested polymerase chain reaction, Vet. Parasitol., 101(1):9-21.

Renard, G., Garcia, J.F., Cardoso, F.C., Richter, M.F., Sakanari, J.A., Ozaki, L.S., Termignoni, C. & Masuda, A. (2000). Cloning and functional expression of a Boophilus microplus cathepsin L-like enzyme, Insect Biochem. Mol. Biol 30(11):1017-26.

Passos, D.T., Ferreira, C.A.S., da Silva, S.S., Richter, M.F. & Ozaki, L.S. (1999). Detection of genomic variability in different populations of the cattle tick Boophilus microplus in Southern Brazil, Vet. Parasitol., 87:83-92.

Mafra, C.L., Paula, S.O., Ozaki, L.S., Patarroyo, J.H. (1997). Useful web sites for researchers studying proteins Braz. J. Med. Biol. Res., 30(3): 283-288

Marsiglia, M. R. L.; Lunge, V. R.; Hotzel, I. & Ozaki, L. S. (1997). Development of a combined selective enrichment method and polymerase chain reaction ( PCR ) assay for the sensitive detection of Salmonella in food samples, World J. Microbiol. Biotechnol. 13:649-654.

Krug, L.P, Lunge, V.R., Ikuta, N., Fonseca, A.S.K., Cheinquer, H., Ozaki, L.S. & Barros, S.G.S. (1996). Hepatitis C Virus Genotypes in Southern Brazil, Braz J Med Biol Res. 29(12): 1629-1632.

Canal, C.W., Maia, H.M., Vaz Junior, I.S., Chies, J.M., Farias, N.A., Masuda, A., Gonzales, J.C., Ozaki, L.S., Dewes, H. (1995) Changing patterns of vitellin-related peptides during development of the cattle tick Boophilus microplus , Exp Appl. Acarol., 19(6) : 325-336.

Hotzel, I., Kabakoff, R. & Ozaki, L.S. (1995). Small extrachromosomal Nucleic Acids in Protozoan Parasites, Ninth International Veterinary Hemoparasite Disease Conference, Merida, Mexico, Veterinary Parasitol., 52:71-78.

Loguercio, L.L., Termignoni, R.R. and Ozaki, L.S. (1994). The reversible inhibition of pollen germination of Nicotiana tabacum L.: an entry into a transformation system, Plant Cell Reports, 13:231-236.

Lunge, V.R., Ikuta, N., Hirigoyen, D., Stoll, M., Bonatto, S. & Ozaki, L.S. (1994). Identification and interrelationship analysis of Bradyrhizobium japonicum strains by Restriction Fragment Length Polymorphism (RFLP) and Random Amplified Polymorphic DNA (RAPD), World J. Microbiol. & Biotechnol., 10:648-652.

Silva Vaz, Jr. I., Ozaki, L.S. and Masuda, A. (1994). Serum of Boophilus microplus infested cattle reacts with different tick tissues, Vet. Parasitol., 52:71-78.

Ikuta, N., Kimura, E. A., Pereira da Silva, L., Debes, A.A. and Ozaki, L.S. (1992). Non-phenolic method of DNA purification from small blood samples and other biological sources suitable for DNA modification and restriction enzymes, Mem. Inst. Oswaldo Cruz, 87(4):589-591.

Johnston, R.C., Rosa Farias, N.A., Gonzalez, J.C., Dewes, H., Masuda, A., Termignoni, C., Amako, K. and Ozaki, L.S. (1990). A putative RNA virus in Babesia bovis . Mol. Biochem. Parasitol., 45:155-158.

Minoprio, P., Andrade, L., Lembezat, M.-P., Ozaki, L.S. and Coutinho, A. (1989). Indiscriminate representation of VH- gene families in the murine B lymphocyte responses to Trypanosoma cruzi . J. Immunol., 142:4017-4021.

Udomsangpetch R., Carlsson J., Wahlin B., Holmquist G., Ozaki LS., Scherf A., Mattei D., Mercereau-Puijalon O., Uni S., Aikawa M. (1989). Reactivity of the human monoclonal antibody 33G2 with repeated sequences of three distinct Plasmodium falciparum antigens. J. Immunol . , 142(10): 3620-3626.

Bonnefoy, S., Mattei, D., Dubremetz, J.F., Guillotte, M., Jouin, H., Ozaki,L.S., Sibilli, L., Mercereau-Puijalon, O. (1988). Plasmodium falciparum : molecular analysis of a putative protective antigen, the thermostable 96-kDa protein. Exp. Parasitol ., 65(1):69-83

Mattei D., Ozaki L.S., da Silva L.P. (1988). A Plasmodium falciparum gene encoding a heat shock-like antigen related to the rat 78 Kd glucose-regulated protein. Nucl. Acids Res. , 16(11):5204.

Kahane, B., Sibili, L., Scherf, A., Jaureguiberry, G., Langsley, G., Ozaki, L.S., Guillote, M., Muller-Hill, B., Pereira da Silva, L. and Mercereau-Puijalon, O. (1987). The polymorphic 11.1 locus of Plasmodium falciparum . Mol. Biochem. Parasitol., 26:77-86.

Altaba i Ruiz, A., Ozaki, L.S., Gwadz, R., Godson, G.N. (1987). Organization and expression of the circumsporozoite (CS) protein gene. Mol. Biochem. Parasitol., 23:233- 245.

Ruiz i Altaba, A., Ozaki, L.S., Zavala, F. and Godson G.N. (1986). Expression of the Plasmodium knowlesi circumsporozoite antigen in Escherichia coli directed by Plasmodium bacterial-like promoter sequences. Gene, 41:135-144.

Ozaki, L.S., Mattei, D., Druhile, P., Blisnick, T., Guillote, M., Puijalon, O. and Pereira da Silva, L. (1986). Plaque antibody selection: rapid immunological analysis of a large number of recombinant phage clones positive to sera raised against Plasmodium falciparum antigens. J. Immunol. Methods, 89:213-219.

Lupski, J.R., Projan, S.J., Ozaki, L.S. and Godson, G.N. (1986). A temperature-dependent pBR322 copy number mutant resulting from a Tn 5 position effect. Proc. Natl. Acad. USA, 83:7381-7385.

Ozaki, L.S., Gwadz, R.W. and Godson, G.N. (1984). Simple centrifugation method for rapid separation of sporozoites from mosquitoes. J. Parasitol., 70(5):831-833.

Lupski, J.R., Gershon, P., Ozaki, L.S. and Godson, G.N. (1984). Specificity of Tn 5 insertions into a 36 nucleotide DNA sequence repeated in tandem seven times. Gene, 30:99- 106.

Godson, G.N., Ellis, J., Lupski, J.R., Ozaki, L.S. and Svec, P. (1984). Molecular biology of Plasmodium knowlesi sporozoites: cloning and expression of the surface antigen gene. Mol. Parasitol., pp. 127-142.

Lupski, J.R., Ozaki, L.S., Ellis, J. and Godson, G.N. (1983). Localization of a Plasmodium surface antigen epitope by Tn5 mutagenesis mapping of a recombinant cDNA clone. Science, 220:1285-1288.

Ozaki, L.S., Svec, P., Nussenzweig, R.S. Nussenzweig, V. and Godson, G.N. (1983). Structure of the Plasmodium knowlesi gene coding for the circumsporozoite protein. Cell, 34:815-822.

Ellis, J., Ozaki, L.S., Gwadz, R.W., Cochrane, A.H., Nussenzweig, V., Nussenzweig, R.S. and Godson, G.N. (1983). Cloning and expression in E. coli of the malarial sporozoite surface antigen from Plasmodium knowlesi . Nature, 302:536-538.

Ozaki, L.S., Kimura, A., Shimada, K. and Takagi, Y. (1982). ColE1 vectors for direct selection of cells carrying a hybrid plasmid. J. Biochem. (Japan), 91:115- 1162.

Ozaki, L.S. (1981). Development of ColE1 vectors for the direct selection of cells carrying a hybrid plasmid. Ph.D. Thesis, Kyushu University, School of Medicine, Department of Biochemistry, Fukuoka, Japan.

Ozaki, L.S., Maeda, S., Shimada, K. and Takagi, Y. (1980). A Novel ColE1::Tn 3 plasmid vector that allows direct selection of hybrid clones in E. coli . Gene, 8:301-314.

Viscardi, R.C., Ozaki, L.S., Morel, C. (1975) Different sensitivities of avian- and mammal-haemoglobin synthesis to elevated temperatures Mol Biol Rep 2(1) : 41-47.

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