Publications

Peer-Reviewed Papers

  1. Marconi, R.T. and W.E. Hill.  1988. Identification of sequences in domain V of Escherichia coli 23S rRNA in the 50S subunit accessible for hybridization with oligonucleotides.  Nucleic Acids Res. 16:1603-1615.
  2. Marconi, R.T. and W.E. Hill. 1989.  Evidence for a tRNA/rRNA interaction site within the peptidyltransferase center of the Escherichia coli ribosome.  Biochem. 28:893-899.
  3. Marconi, R.T., Lodmell, J.S. and W.E. Hill. 1990. Identification of a rRNA/chloramphenicol interaction site in the peptidyltransferase center of the 50S subunit of the Escherichia coli ribosome.  J. Biol. Chem. 265:7894-7899.
  4. Marconi, R., Wigboldus, J., Weissbach, H. and N. Brot. 1991. Transcriptional start and metR binding sites on the Escherichia coli metH gene.  Biochem. Biophys. Res. Comm. 175:1057-1063.
  5. Marconi, R.T. and C.F. Garon. 1992.   Phylogenetic analysis of the genus Borrelia:  a comparison of North American and European isolates of Borrelia burgdorferi.  J. Bacteriol. 174:241-244.
  6. Marconi, R.T. and C.F. Garon. 1992. Identification of a third genomic group of Borrelia burgdorferi through signature nucleotide analysis and 16S rRNA sequence determination.  J. Gen. Micro. 138:533-536.
  7. Marconi, R.T., Lubke, L., Hauglum, W. and C.F. Garon. 1992. Species-specific identification of and distinction between Borrelia burgdorferi genomic groups by using 16S rRNA-directed oligo probes.  J. Clin. Microbiol. 30:628-632.
  8. Marconi, R.T. and C.F. Garon. 1992. Development of polymerase chain reaction primer sets for diagnosis of Lyme disease and for species-specific identification of Lyme disease isolates by 16S rRNA signature nucleotide analysis. J. Clin. Microbiol. 30:2830-2834.
  9. Marconi, R.T., Samuels, D.S. and C.F. Garon.  1993. Transcriptional analyses and mapping of the ospC gene in Lyme disease spirochetes.  J. Bacteriol. 175:926-932.
  10. Marconi, R.T., Konkel, M.E. and C.F. Garon. 1993. Variability of the osp gene products found among the species of Lyme disease spirochetes.  Infect. Immun. 61:2611-2617.
  11. Marconi, R.T., Samuels, D.S., Schwan, T.G. and C.F. Garon. 1993.   Identification of a protein in several species of Borrelia related to OspC of the Lyme spirochetes.  J. Clin. Microbiol. 31:2577-2583.
  12. Samuels, D.S., Marconi, R.T. and C.F. Garon. 1993. Variation in the size of the ospA-containing linear plasmid and the linear chromosome among the three Borrelia species associated with Lyme disease.  J. Gen. Microbiol. 139:2445-2449.
  13. Samuels, D.S., Marconi, R.T., Huang, W.M. and C.F. Garon. 1994. Mutations in gyrB from variants of Borrelia burgdorferi resistant to coumermycin A1.  J. Bacteriol. 136:3072-3075.
  14. Konkel, M.E., Marconi, R.T., Mead, D.J. and W. Cieplak. 1994. Cloning, sequencing, and heterologous expression of a gene encoding HU from Campylobacter jejuni. Gene. 146:83-86.
  15. Marconi, R.T., Samuels, D.S. and C.F. Garon. 1994.  Analysis of the distribution and molecular heterogeneity of the ospD gene among the Lyme disease spirochetes: Evidence for lateral gene exchange.  J. Bacteriol. 176:4752-4582.
  16. Feir, D., Santanello, C.R., Li, B.W., Xie, C.S., Masters, E., Marconi, R. and G. Weil. 1994. Evidence supporting the presence of Borrelia burgdorferi in Missouri.  J. Trop. Med. Hyg. 51:475-482.
  17. Konkel, M.E., Marconi, R.T., Mead, D.J. and W. Cieplak. 1994. Identification of an intervening sequence within the 23S rRNA genes of Campylobacter jejuni.  Mol. Microbiol.  14:235-241.
  18. Marconi, R.T., Liveris, D. and I. Schwartz. 1995. Identification of novel insertion elements, RFLP patterns, and discontinuous 23S rRNA in Lyme disease spirochetes: Phylogenetic analyses of rRNA genes and their intergenic spacers in Borrelia japonica sp. nov. and genomic group 21038 (Borrelia andersonii sp. nov.) isolates. J. Clin. Microbiol.  33:2427-2434.
  19. Marconi, R.T., Casjens, S., Munderloh, U.G. and D.S. Samuels. 1996.  Analysis of atypical linear plasmids in Borrelia burgdorferi sensu lato isolates: Implications concerning the potential mechanism of linear plasmid replication. J Bacteriol. 178:3357-3361.
  20. Marconi. R.T., Sung, S.Y., Carlyon, J. and C. Norton-Hughes. 1996. Molecular and evolutionary analyses of a variable series of genes in Borrelia burgdorferi that are related to ospE and ospF, comprise a gene family, and that share a common upstream homology box.   J.  Bacteriol. 178:5615-5626.
  21. Cloud, J.L., Marconi, R.T., Garon, C.F., Tilly, K. and D.S. Samuels. 1997. Cloning and expression of the Borrelia burgdorferi lon gene.  Gene. 194:137-141.
  22. Carlyon, J., LaVoie, C., Sung, S.Y. and R.T.  Marconi. 1998. Analysis of the organization of multicopy, linear and circular plasmid carried ORFs in Borrelia burgdorferi sensu lato isolates.  Infect. Immun.66:1149-1158.
  23. Sung, S.Y., LaVoie, C., Carlyon, J. and R.T. Marconi. 1998. Genetic divergence and evolutionary instability in ospE related members of the UHB gene family in Borrelia burgdorferi sensu lato complex isolates.  Infect. Immun. 180:4974-4981.
  24. Carlyon, J.A. and R.T. Marconi. 1998. Cloning and molecular characterization of a multi-copy, linear plasmid carried, repeat motif containing gene from Borrelia turicatae, a causative agent of Relapsing fever.  J. Bacteriol. 66:4656-4668.
  25. Marconi, R.T., Hohenberger, S., Jauris-Heipke, S., Schulte-Spechtel, U., LaVoie, C.P., Rößler, D. and Wilske, B. 1999. Genetic analysis of Borrelia garinii OspA serotype 4 strains associated with neuroborreliosis: evidence for extensive genetic homogeneity.  J Clin Microbiol.  37:3965-3970.
  26. Carlyon, J.A., Roberts, D.M. and R.T. Marconi. 2000. Evolutionary and molecular analyses of the Borrelia bdr super gene family: Delineation of distinct sub-families and demonstration of the genus wide conservation of putative functional domains, structural properties and repeat motifs. Microbial Pathogenesis.28:89-105.
  27. Sung, S.Y., McDowell, J., Carlyon, J.A. and R.T. Marconi. 2000. Mutation and recombination in the UHB flanked ospE-related genes of the Lyme disease spirochetes results in the development of new antigenic variants during infection. Infection and Immunity. 68:1319-1327.
  28. Carlyon, J.A., Roberts, D.M. and R.T. Marconi. 2000. Molecular and immunological analyses of the Borrelia turicatae Bdr protein family. Infect Immun. 68:2369-2373.
  29. Roberts, D.M., Theisen, M., Carlyon, J.A. and R.T. Marconi. 2000. The bdr gene families of the Lyme disease and relapsing fever spirochetes: possible influence on biology, pathogenesis and evolution. Emerg Infectious Dis. 6:110-122.
  30. Roberts, D.M., Theisen, M. and R.T. Marconi. 2000. Members of the Bdr protein family exhibit variable cellular localization in the Lyme disease and relapsing fever Borrelia. J. Bacteriol.182:4222-4226.
  31. McDowell, J.V., Sung, S.Y, and R.T. Marconi. 2001. Analysis of the mechanisms associated with the loss of infectivity of clonal populations of Borrelia burgdorferi B31MI. Infect. Immun. 69:3670-3677.
  32. Sung, S.Y, McDowell, J.V. and R.T. Marconi. 2001. Evidence for the contribution of point mutations to vlsE variation and for apparent constraints on the net accumulation of sequence changes in vlsE during infection with Lyme disease spirochetes. J. Bacteriol. 183:5855-5861.
  33. McDowell, J.V., Sung, S.Y., Price, G. and R.T. Marconi. 2011. Demonstration of the genetic stability and temporal expression of select members of the Lyme disease spirochete OspF protein family during infection in mice. Infect. Immun. 69:4831-4838.
  34. McDowell, J.V., Sung, S.Y., Hu, L.T. and R.T. Marconi. 2002. Evidence that the variable regions of the central domain of VlsE are antigenic during infection with the Lyme disease spirochetes. Infect. Immun. 70:4196-4203.
  35. Roberts, D.M., Caimano, M., Radolf, J.R., Nelson, D. and R.T. Marconi. 2002. Environmental and differential expression of the Bdr Protein family of the Lyme disease spirochetes. Infect. Immun. 70:7033-41.
  36. Metts, M.S., McDowell, J.V., Theisen, M., Hansen, P.R. and R.T. Marconi. 2003. Analysis of the OspE determinants involved in the binding of factor H and OspE targeting antibodies elicited during Borrelia burgdorferi infection in mice. Infect. Immun. 71:3587-96.
  37. McDowell, J.V., Tran, E., Hamilton, D., Metts, M.S., Wolfgang, J. and R.T. Marconi. 2003.  Comprehensive analysis of the factor H binding capabilities of Borrelia species associated with Lyme disease: Demonstration of differential binding and delineation of distinct classes of factor H binding proteins. Infect. Immun.71:3597-602.
  38. McDowell, J.V., Tran, E., Hamilton, D., Wolfgang, J., Miller, K. and R.T. Marconi. 2003. Analysis of the ability of spirochete species associated with relapsing fever, avian borreliosis, and epizootic bovine abortion to bind factor H and cleave C3b. J. Clin. Microbiol. 41:3905-3910.
  39. Miller, K., McDowell, J.V., Atkins, L. and R.T. Marconi. 2004. Identification and characterization of a linear plasmid encoded factor H binding protein (FhbA) of the Relapsing Fever Spirochete, Borrelia hermsii. J. Bacteriol. 186:2612-2618.
  40. McDowell, J.V., Wolfgang, J., Senty, L., Sundy, C.M. Noto, M.J. and R.T. Marconi. 2004. Demonstration of the involvement of OspE coiled-coil structural domains and higher order structural elements in the binding of infection induced antibody and the complement regulatory protein, factor H. J. Immunology. 173:7471-7480.
  41. Zhang, H.M., Raji, A., Theisen, M., Hansen, P.R. and R.T. Marconi. 2005. bdrF2 of the Lyme disease spirochetes is co-expressed with a series of cytoplasmic proteins and produced specifically during early infection.  J. Bacteriol. 187:175-184.
  42. McDowell, J.V., Harling, M.E., Rogers, E. and R.T. Marconi. 2005. Putative coiled-coil structural elements of the BBA68 protein of the Lyme Disease Spirochetes are required for formation of its factor H binding site. J Bacteriol.  187:1317-1323.
  43. Earnhart, C.G., Dumler, J.S. and R.T. Marconi. 2005. Demonstration of OspC type diversity in invasive human Lyme disease isolates and identification of previously uncharacterized epitopes that define the specificity of the OspC antibody response. Infect. Immun.73:7869-7877. 
  44. Zhang, H. and R. T. Marconi. Demonstration of co-transcription of a 30 gene operon from a 32 kb circular plasmid of Borrelia burgdorferi that is induced by the DNA alkylating agent, MNNG: Additional evidence for the existence of bacteriophage. J. Bacteriol. 187:7895-7995. 2005.
  45. McDowell, J.V., Lankford, J., Stamm, L., Sadlon, T., Gordon, D.L. and R.T. Marconi. 2005. Demonstration of factor H Binding to the FhbB protein of Treponema denticola, a Pathogen Associated with Periodontal Disease in humans. Infect. Immun. 73:7126-7132.
  46. McDowell, J.V., Hovis, K.M. and R.T. Marconi. 2006. Evidence that the factor H binding BBA68 (BbCRASP-1) protein of the Lyme disease spirochetes does not contribute to factor H mediated immune evasion in humans and other animals. Infect. Immun. 74:3030-4.
  47. Hovis, K.M., Tran, E., Sundy, C.M., Buckles, E., McDowell, J.V. and R.T. Marconi. 2006. Selective binding of Borrelia burgdorferi OspE Paralogs to Factor H and Serum Proteins from Diverse Animals: possible expansion of the role of OspE in Lyme disease pathogenesis. Infect. Immun. 74:1967-72.
  48. Hovis, K.M., Sadlon, T., Raval, G., Gordon, D.L. and R.T. Marconi. 2006. Molecular analyses of the interaction of Borrelia hermsii FhbA with the complement regulatory protein factor H and infection induced antibody. Infect. Immun. 74:2007-14.
  49. Hovis, K.M., Schriefer, M.E., Bahlani, S. and R.T. Marconi. 2006. Immunological analyses of the Borrelia hermsii factor H/FHL-1 binding protein, FhbA: Demonstration of its utility as a diagnostic marker and epidemiological tool for tick-borne relapsing fever. Infect. Immunity. 74:4519-4529.
  50. Buckles, E., Earnhart, C.G. and R.T. Marconi. 2006. Analysis of the antibody response in humans to the type A OspC loop 5 domain and assessment of the potential utility of the loop 5 epitope in Lyme disease vaccine development. Clinical and Vaccine Immunology. 13(10):1162-5.
  51. Earnhart, C.G., Buckles, E. and R.T. Marconi. 2007. Development of an OspC-based tetravalent, recombinant, chimeric vaccinogen that elicits bactericidal antibody against diverse Lyme disease spirochete strains.  Vaccine. 25:466-480.
  52. McDowell, J.V., Frederick, J. and R.T. Marconi. 2007. Identification of the gene encoding the FhbB protein of Treponema denticola, a unique FHL-1 binding protein. Infect Immunity. 75:1050-54.                                                                
  53. Earnhart, C.G. and R.T. Marconi. 2007. Construction and analysis of variants of a polyvalent Lyme disease vaccine: Approaches for improving the immune response to chimeric vaccinogens. Vaccine.  25:3419-3427.
  54. Earnhart, C.G. and R.T. Marconi. 2007. OspC phylogenetic analyses support the feasibility of a broadly protective polyvalent chimeric Lyme disease vaccine. Clinical and Vaccine Immunology. 14:628-634.
  55. Earnhart, C.G. and R.T. Marconi. 2007. An octavalent Lyme disease vaccinogen induces antibodies that recognize all incorporated OspC type-specific sequences Human Vaccines. 3(5).
  56. Rogers, E.A. and R.T. Marconi. 2007. Delineation of species-specific binding properties of the CspZ protein (BBH06) of Lyme disease spirochetes: evidence for new contributions to the pathogenesis of Borrelia spp. Infect Immun. 75:5272-81.
  57. McDowell, J.V., Frederick, J., Stamm, L. and R.T. Marconi. 2007. Identification of the gene encoding the FhbB protein of Treponema denticola, a highly unique factor H-like protein 1 binding protein. Infect Immun. 75:1050-4.
  58. Caswell, C.C., Han, R., Hovis, K.M., Ciborowski, P., Keene, D.R., Marconi, R.T.  and S. Lukomski. 2008. The Scl1 protein of M6-type group A Streptococcus binds the human complement regulatory protein, factor H, and inhibits the alternative pathway of complement. Mol Microbiol. 67:584-96.
  59. Hovis, K.M., Freedman, J.C., Zhang, H.M., Forbes, J.L. and R.T. Marconi. 2008. Identification of an antiparallel coiled-coil/loop domain required for ligand binding by the Borrelia hermsii FhbA protein: additional evidence for the role of FhbA in the host-pathogen interaction. Infect Immun. 76:2113-22.
  60. Frederick, J.R., Rogers, E.A. and R.T. Marconi. 2008. Analysis of a growth-phase-regulated two-component regulatory system in the periodontal pathogen Treponema denticola. J Bacteriol. 190:6162-9.
  61. Freedman, J.C., Rogers, E.A., Kostick, J.L., Zhang, H.M., Iyer, R., Schwartz, I. and R.T. Marconi. 2009. Identification and molecular characterization of a cyclic-di-GMP effector protein, PlzA (BB0733): additional evidence for the existence of a functional cyclic-di-GMP regulatory network in the Lyme disease spirochete, Borrelia burgdorferi. FEMS Immunol Med Microbiol. 58:285-294.
  62. McDowell, J.V., Huang, B., Fenno, J.C. and R.T. Marconi. 2009. Analysis of a unique interaction between the complement regulatory protein factor H and the periodontal pathogen Treponema denticola. Infect Immun. 77:1417-25.
  63. Rogers, E.A., Abdunnur, S.V., McDowell, J.V. and R.T. Marconi. 2009. Comparative analysis of the properties and ligand binding characteristics of CspZ, a factor H binding protein, derived from Borrelia burgdorferi isolates of human origin. Infect Immun. 77:4396-4405.
  64. Rogers, E.A., Terekhova, D., Zhang, H.M., Hovis, K.M., Schwartz, I. and R.T. Marconi. 2009. Rrp1, a cyclic-di-GMP-producing response regulator, is an important regulator of Borrelia burgdorferi core cellular functions. Mol Microbiol. 71:1551-1573.
  65. Earnhart, C.G., Leblanc, D.V., Alix, K.E., Desrosiers, D.C., Radolf, J.D. and R.T. Marconi. 2010. Identification of residues within ligand-binding domain 1 (LBD1) of the Borrelia burgdorferi OspC protein required for function in the mammalian environment. Mol Microbiol. 76:393-408.
  66. Sarkar, J., Frederick, J. and R.T. Marconi. 2010. The Hpk2-Rrp2 two-component regulatory system of Treponema denticola:  a potential regulator of environmental and adaptive responses. Mol Oral Microbiol. 25:241-251.
  67. McDowell, J.V., Frederick, J., Miller, D.P., Goetting-Minesky, M.P., Goodman, H., Fenno, J.C. and R.T. Marconi. 2011. Identification of the primary mechanism of complement evasion by the periodontal pathogen, Treponema denticola. Mol Oral Microbiol. 26:140-149.
  68. Earnhart, C.G., Rhodes, D.V. and R.T. Marconi. 2011. Disulfide-Mediated Oligomer Formation in Borrelia burgdorferi Outer Surface Protein C, a Critical Virulence Factor and Potential Lyme Disease Vaccine Candidate.  Clin Vaccine Immunol. 18:901-6.
  69. Kostick, J.L., Szkotnicki, L.T., Rogers, E. A., Bocci, P., Raffaelli, N. and R.T. Marconi. 2011. The diguanylate cyclase, Rrp1, regulates critical steps in the enzootic cycle of the Lyme disease spirochetes.  Mol. Microbiol, 81:219-31.
  70. Fine, L.M., Earnhart, C.G. and R.T. Marconi. 2011. Genetic Transformation of the Relapsing Fever Spirochete Borrelia hermsii: Stable Integration and Expression of Green Fluorescent Protein from Linear Plasmid 200.  J Bacteriol. 193:3241-5.
  71. Miller, D.P., McDowell, J.V., Bell, J.K. and R.T. Marconi. 2011. Crystallization of the Factor H binding protein, FhbB, of the periopathogen Treponema denticola. Acta Crystallographica. 67:678-81.
  72. Frederick, J. and R.T. Marconi. 2011. Genetic regulatory mechanisms of the periopathogen, Treponema denticola. J Dental Res. In press.
  73. McDowell, J.V. and R.T. Marconi. 2011. Tick salivary proteins offer the Lyme disease spirochetes an easy ride and another way to hide. Cell Host and Microbe.

BOOK CHAPTERS

  1. Hill, W.E., Gluick, T., Marconi, R.T., Merryman, C., Tapprich, W., Tassanakajohn, A. and J.W. Weller. 1990. Probing ribosome structure and function using short complementary DNA oligomers.  p. 253-261.  In Hill, W., Dahlberg, A., Garrett, R., Moore, P., Schlessinger, D., and Warner, J. (eds.) in The Ribosome:  Structure, Function and Evolution.  ASM Press, Washington, D.C.
  2. Earnhart, C., and R.T. Marconi. 2009. Lyme disease. p. 1031-1060. In A. D. Barrett and L. R. Stanberry (ed.), Vaccines for Biodefense and Emerging and Neglected Diseases. Elsevier, San Diego, CA.
  3. Marconi, R.T. and C.G. Earnhart. 2010. Lyme disease vaccines. p. 467-486. In D.S. Samuels and J.D. Radolf (Editors). Borrelia molecular biology, host interaction and pathogenesis. Caister Academic Press, New York, NY.

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