Techniques in
Neuroscience and Cell Biology (ANAT 615)
Spring 2008
Tuesday/Thursday
Sanger Hall,
Rm 1-067
Techniques
in Neuroscience and Cell Biology is a two credit hour course offered in the
Spring semester and is designed to introduce the student to techniques and
protocols used in neuroscience and cell biology. Topics will be covered in a lecture format
and grades will be assigned as Pass/Fail based on attendance. Pre-requisite is Biochemistry, Cell and
Molecular Biology (BIOC 503/504) or equivalent.
January 15 Introduction
Dr.
January 17 Subcellular fractionation and protein analysis Dr.
January 22 Subcellular fractionation and protein analysis Dr.
January 24 Gel
electrophoresis and Western blotting Dr.
January 29 Gel
electrophoresis and Western blotting Dr.
January 31 Gene
arrays Dr.
Mike Miles
February 5 Proteomic
analysis Dr.
February 7 Molecular
approaches to the study of the nervous system Dr.
Babette Fuss
February 12 Molecular
approaches to the study of the nervous system Dr.
Babette Fuss
February 14 Genetic
manipulation of protein expression Dr. Jameel
Dennis
February 19 Genetic
manipulation of protein expression Dr.
Jameel Dennis
February 21 Transgenic
and knockout models in neuroscience Dr. Jolene Windle
February 26 Microscopy Basics &
Sample preparation (LM & EM) Dr. Scott Henderson
February 28 Molecular Localization (ICC;
ISH; Fluorescent proteins) Dr. Scott Henderson
March 4 Fluorescence-based imaging
& Confocal Microscopy Dr.
Scott Henderson
March 6 Advanced Microscopic
Imaging: 2-photon, TIRF Dr. Scott Henderson
March 18 Quantitative Imaging, Image
Processing & Analysis Dr. Scott Henderson
March 20 Anatomical tracing Dr.
March 25 Culturing neurons
and glial cells Dr.
March 27 Overview
of techniques in electrophysiology Dr. Kimberle Jacobs
April 1 Preparations
in electrophysiology Dr. Tom Reeves
April 3 Single and
multi-unit extracellular recording Dr. Alex Meredith
April 8 Sharp intracellular recording Dr. Les
Satin
April 10 Patch
clamp recording Dr.
Rory McQuiston
April 15 Behavioral
testing Dr.
Robert Hamm
April 17 Behavioral
testing Dr.
Robert Hamm
April 22 In vivo imaging Dr.
Panos Fatouros
April 24 TBA
April 29 TBA
For more information contact: Dr.
Department
of Anatomy and Neurobiology, Rm 9-053 Phone: 828-0948
Email: jbigbee@vcu.edu