• The
Mass Spectrometry Analysis Request Form must be properly completed
and Budget Code provided before any service can be rendered by the
Resource.
• Optimum amounts
of samples in solution depend on the mass spectrometric technique
used, but should preferably be 1 µM or greater, in volatile/protic
solvents. Preferred solvents include: MeOH, MeOH/H2O, ACN/H2O
(All solvents in 0.1% formic or acetic acid). DMF, DMSO and large
polar solvents are NOT suitable for mass spectrometric analyses.
• All samples should be free of non-volatile
buffers, detergents, salts, urea, glycerol, chaps, etc. These impurities
are detrimental to mass spectrometric analyses.
• Preferred buffers include: 0.1% formic acid,
0.1% acetic acid, 10mM ammonium acetate, 1-10mM ammonium formate,
0.1% TFA and 1-10mM ammonium bicarbonate. Less than 2% triethylamine
is suitable for oligonucleotides.
