Introduction to Bioinformatics (Fall 2007)
Questionnaire on notes: Protein Overexpression and Protein Threading
(please press SUBMIT button when finished)

I. Basic Information

A. Your name 
II. Overview of protein expression in a heterologous host
A. How do you feel about your understanding of the general issues surrounding protein overexpression? 

B. How do you feel about your understanding of transcriptional fusions? 
 
Comments for questions II.A through II.B:

III. The Scenario
A. Do you feel comfortable about your understanding of why overexpression of UDP-glucose dehydrogenase might be a good thing? 

B. Do you feel comfortable about what random mutagenesis is for and how PCR-mediated random mutagenesis works? 

C. Do you feel comfortable about the nature and role of the translational fusion of UDP-glucose dehydrogenase to GFP? 
 
Comments for questions III.A through II.C:

IV. Implementation of the plan to display an approximate structure for UDPGD
A. Which of the following were you able to accomplish?
Get amino acid sequence of UDPGD  Get amino acid sequence of similar protein 
Get PDB file of similar protein  Run Protein Explorer  Download and run Clustal X
Comments for question IV:
III. Miscellaneous
A. Please choose up to four study questions from the notes that you would most like discussed in class:
SQ1 SQ2 SQ3 SQ4 SQ5 SQ6 SQ7 SQ8
B. Put here any miscellaneous comments, questions, suggestions, concerns you may have.
 

Thanks!

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