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Jack L. Haar, Ph.D.
Professor of Anatomy & Neurobiology
B.S., Biology, Capital University (1964)
M.S., Zoology, University of Arizona (1966)
Ph.D., Anatomy, Ohio State University (1970)
| Office Address: | | Sanger Hall |
| | | Room 9-064 |
| | | Medical Campus of Virginia Commonwealth University |
| | | Box 980709 | | | | Richmond, VA
23298-0709 | | Office
Phone: | | (804) 828-3831 | | FAX: | | (804) 828-9477 | | e-mail: | | jlhaar@vcu.edu |
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RESEARCH AND SCHOLARLY INTERESTS
My research interest is focused primarily on embryonic and fetal blood cell formation in mice. Two
frequently cited papers from my early work describe the morphologic events that occur in the mouse
yolk sac, the first site of mammalian hematopoiesis in mammals (1, 2). Primitive cells formed in the
yolk sac migrate to the thymus, proliferate and differentiate into T-lymphocytes. The events involved
with the cell migration aspect of this process have been studied in my laboratory using in vitro
migration assays, immunological assays, FACS analysis and sorting, and cell culture.
My current research effort focuses on isolating very early erythrocytes from frozen sections of E9 and
E10 mouse yolk sac using laser capture microdissection. Confirmation that enriched cell populations
have been collected is done using quantitative RT-PCR. RNA from these cells is then used to
establish tissue- and stage-specific gene expression profiles by hybridizing the RNA to an Affymetrix
GeneChip ® 430 2.0 array for microarray analysis. The purpose of the research is to identify novel
erythroid-specific genes that are critical in primitive erythropoiesis.
REPRESENTATIVE PUBLICATIONS
Haar J.L., L. Redmond, P. Basu and J. Lloyd: Enrichment of erythroid cells from mouse embryonic yolk
sac and fetal liver using laser capture microdissection (LCM). FASEB J. 19 (4):A804. 2005 (abstract)
Basu P, P.E. Morris, J.L. Haar, M.A. Wani, J.B. Lingrel, K.M.Gaensler and J.A. Lloyd. KLF2 is
essential for primitive erythropoiesis and regulates the human and murine embryonic beta-like
globin genes in vivo. Blood, 106(7):2566-71, 2005
L.C. Redmond, J.L. Haar, M.L. Giebel, C.I. Dumur, P. Basu, J.L. Ware and J.A. Lloyd. Isolation of erythroid cells
from the mouse embryonic yolk sac by laser capture microdissection and subsequent microarray hybridization.
Blood Cells Mol Dis., 37(1):27-32, 2006
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